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JoVE Journal
Biology

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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
 

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Article DOI: 10.3791/2812-v 06:09 min July 31st, 2011
July 31st, 2011

Chapters

Summary

Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) couples affinity enrichment of peptides with stable isotope dilution mass spectrometry (MRM-MS) to provide quantitative measurement of peptides as surrogates for their respective proteins. Here we describe the protocol using magnetic particles in a partially automated format.

Tags

Quantification Proteins Peptide Immunoaffinity Enrichment Mass Spectrometry Traditional Immunoassays SISCAPA Stable Isotope Standards Capture By Anti-peptide Antibodies SRM/MRM-MS Small Molecules Protein Concentration Proteotypic Peptides Trypsin
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