Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

ReAsH / FLASH标签和图像分析Tetracysteine​​传感器蛋白在细胞中
 
Click here for the English version

ReAsH / FLASH标签和图像分析Tetracysteine​​传感器蛋白在细胞中

Article DOI: 10.3791/2857-v 12:20 min August 31st, 2011
August 31st, 2011

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

biarsenical染料Flash和ReAsH特异性结合蛋白质tetracysteine​​图案,并在活细胞中的蛋白质可以选择性地标签。最近这个标签的战略已被用于开发传感器用于不同的蛋白质的构象或低聚国家。我们描述了标签的方式和方法,定量分析具有约束力。

Tags

细胞生物学,第54期,tetracysteine​​,训练班,ReAsH,FLASH,biarsenical染料,荧光,成像,共聚焦显微镜,ImageJ,绿色荧光蛋白
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter