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Biology

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The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation
 

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation

Article DOI: 10.3791/2874-v 07:20 min June 10th, 2011
June 10th, 2011

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Summary

A tissue preparation is described for visualization and experimental manipulation of the living microcirculation. In anesthetized male mice, the thin, highly vascularized cremaster muscle is prepared for intravital microscopy to study microvascular networks including arterioles, capillaries and venules. This preparation is readily adapted for rats and hamsters.

Tags

Mouse Cremaster Muscle Preparation Intravital Imaging Microcirculation Homeostasis Blood Supply Arterioles Capillaries Venules Oxygenated Blood Diffusional Exchange Experimental Approach Visualizing Manipulating Inflammation Histology Electron Microscopy Vasoactive Drugs Reflected Light
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