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在富含GC KISS1受体序列与人类生殖系统紊乱中确定的诱变和基因突变分析
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在富含GC KISS1受体序列与人类生殖系统紊乱中确定的诱变和基因突变分析

Article DOI: 10.3791/2897-v • 12:50 min • September 4th, 2011
September 4th, 2011 •
1, 1, 1,2
1Division of Endocrinology and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, 2Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine

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kisspeptin受体（KISS1R）的突变与患者的生殖系统紊乱。在这里，我们描述如何引入利益的突变的KISS1R富含GC的序列以及使用KISS1R结构特点免疫沉淀和免疫印迹的受体的降解途径

Tags

55期，GPR54，KISS1R，性早熟，膜受体，蛋白酶，降解，富含GC，定点突变，免疫遗传学，
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