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Mutagenese en analyse van genetische mutaties in het GC-rijke KISS1 Receptor Sequence genoemd in Mensen met voortplantingsstoornissen
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Mutagenese en analyse van genetische mutaties in het GC-rijke KISS1 Receptor Sequence genoemd in Mensen met voortplantingsstoornissen

Article DOI: 10.3791/2897-v • 12:50 min • September 4th, 2011
September 4th, 2011 •
1, 1, 1,2
1Division of Endocrinology and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, 2Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine

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Mutaties in de kisspeptin receptor (KISS1R) zijn geassocieerd met reproductieve stoornissen bij patiënten. Hier beschrijven we hoe de mutaties van belang te introduceren in de GC-rijke sequentie van KISS1R evenals het gebruik van KISS1R constructies om de afbraakroute van de receptor karakteriseren door immunoprecipitatie en western blot

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Genetica GPR54 KISS1R voortijdige puberteit membraan receptor proteasoom afbraak GC-rijke site-directed mutagenese immunoprecipitatie
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