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JoVE Journal
Biology

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农杆菌介导的病毒诱导的基因沉默含量在棉花
 
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农杆菌介导的病毒诱导的基因沉默含量在棉花

Article DOI: 10.3791/2938-v 10:18 min August 20th, 2011
August 20th, 2011

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Summary

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Click here for the English version.

我们目前为农杆菌介导的病毒诱导的基因沉默(VIGS)棉花检测的详细协议。烟草脆裂病毒(TRV)派生的VIGS载体部署GrCLA1​​棉花,Cloroplastos alterados 1基因诱导RNA沉默。白化沉默GrCLA1​​引起的表型观察接种后2个星期内,在苗期。

Tags

植物生物学,第54期,根,棉,功能基因组学,病毒诱导的基因沉默
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