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Agrobacterium-vermittelte Virus-induced gene silencing Assay In Cotton
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Agrobacterium-vermittelte Virus-induced gene silencing Assay In Cotton

Article DOI: 10.3791/2938-v • 10:18 min • August 20th, 2011
August 20th, 2011 •
1, 1, 2, 1
1Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, 2Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology, Texas A&M University

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Wir präsentieren Ihnen die ausführlichen Protokoll für Agrobacterium-vermittelten Virus-induzierte Gen-Silencing (VIGS)-Assay in Baumwolle. Der Tabak Rassel-Virus (TRV)-abgeleiteten Vektoren VIGS wurden eingesetzt, um RNA-Silencing von Baumwolle GrCLA1, Cloroplastos alterados 1-Gens zu induzieren. Der Albino-Phänotyp durch Schweigen GrCLA1 ​​verursacht wurde am Keimlingsstadium innerhalb von 2 Wochen nach der Impfung beobachtet.

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Plant Biology Ausgabe 54 Agrobacterium Cotton Functional Genomics Virus-induced gene silencing
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