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Biology

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Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis
 

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Article DOI: 10.3791/2959-v 07:49 min August 27th, 2011
August 27th, 2011

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Summary

A standard approach to prepare adult Drosophila eyes for semi-thin sectioning and light microscopic analysis is presented here. The protocol can be used for gross morphological analysis of eye defects, or with the indicated adjustments can be used to determine genetic requirements of genes in specific cell types of the eye (e.g. clonal analysis of photoreceptors) or for electron microscopic analysis.

Tags

Drosophila Model System Development Genetically Tractable Mutant Strains Technical Tricks Forward Genetic Studies Reverse Genetic Studies Fly Eye Mutational Analysis Facets Or Ommatidia Rough Eye Phenotype Eye Size Fixation Plastic Embedding Thin-sectioning Eye Imaginal Disc
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