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JoVE Journal
Immunology and Infection

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在艾滋病毒感染者病毒载量低于检测限制标准的临床实验放大和量化HIV - 1 RNA
 
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在艾滋病毒感染者病毒载量低于检测限制标准的临床实验放大和量化HIV - 1 RNA

Article DOI: 10.3791/2960-v 13:59 min September 26th, 2011
September 26th, 2011

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Summary

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Click here for the English version.

量化血浆中HIV - 1 RNA水平和测序单一的HIV - 1病毒载量低于检测限(50-75拷贝/ ml)与个人的基因组是困难的。在这里,我们描述了如何提取和量化使用实时PCR检测方法能够可靠措施,HIV - 1 RNA下降到0.3拷贝/ ml,以及如何放大由单一的基因组测序的病毒基因组病毒载量非常低的样品,血浆病毒RNA。

Tags

免疫学杂志,55期,单基因组测序,SGS,实时PCR,单拷贝检测,政制事务局局长,HIV - 1,超敏感,RNA提取
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