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Journal / Immunology and Infection / Struttura del virus HIV-1 Assemblee Capsidico da…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Struttura del virus HIV-1 Assemblee Capsidico da Cryo-microscopia elettronica e iterativo ricostruzione elicoidale reale spazio
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Struttura del virus HIV-1 Assemblee Capsidico da Cryo-microscopia elettronica e iterativo ricostruzione elicoidale reale spazio

Article DOI: 10.3791/3041-v • 12:39 min • August 9th, 2011
August 9th, 2011 •
1, 1, 1
1Department of Structural Biology, University of Pittsburgh School of Medicine

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Questo articolo descrive un metodo per ottenere un modello tridimensionale (3D) struttura delle molecole elicoidale assemblati con crio-microscopia elettronica. In questo protocollo, utilizziamo gruppi di HIV-1 capside per illustrare la procedura dettagliata ricostruzione 3D per ottenere una mappa della densità del iterativo elicoidale reale spazio metodo di ricostruzione.

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Immunologia Numero 54 crio-microscopia elettronica l'indicizzazione elicoidali elica nello spazio reale ricostruzione assemblee tubolare HIV-1 del capside
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