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JoVE Journal
Biology

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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
 

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Article DOI: 10.3791/3060-v 10:22 min September 21st, 2011
September 21st, 2011

Chapters

Summary

An automated method for identifying suitable hydrophobic interaction chromatography (HIC) media to be used in the process of protein purification is presented. The method utilizes a medium-pressure liquid chromatography system including automated buffer blending, dynamic sample loop injection, sequential column selection, multi-wavelength analysis, and split fraction eluate collection.

Tags

Automated Hydrophobic Interaction Chromatography Protein Purification Chromatographic Medium Selection Scouting Hydrophobicity Affinity Chromatography Ion Exchange Chromatography Protein Concentration Ammonium Sulfate Purification Method Nonpolar Ligand Chromatography Column Salt Concentration
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