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Journal / Bioengineering / Visualization of Recombinant DNA and Protein…
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Bioengineering

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JoVE Journal Bioengineering
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Article DOI: 10.3791/3061-v • 08:30 min • July 18th, 2011
July 18th, 2011 •
1, 2, 2
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University

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Summary
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A tapping mode atomic force microscope (AFM) method for the visualization of plasmid DNA, cytoplasmic proteins, and DNA-protein complexes is described. The method includes alternate approaches for preparing samples for AFM imaging following biochemical manipulation. DNA containing specific protein interacting regions are observed in near-physiologic buffer conditions.

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Atomic Force Microscopy AFM Visualization Recombinant DNA Protein Complexes Nano-scale Probe Elevation Measurement Macromolecules Substrate Surface Electrostatic Forces Topographical Map Three-dimensional Particles Tapping Mode AFM Biomaterials Aqueous Buffers Immunoadsorbed Proteins Glucocorticoid Receptor (GR) Buffered Solution
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