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Journal / Immunology and Infection / IgY Technology: Extraction of Chicken Antibodies…
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Immunology and Infection

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IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

Article DOI: 10.3791/3084-v • 07:26 min • May 1st, 2011
May 1st, 2011 •
1, 2, 3, 4, 5
1Center for Biological Security, Robert Koch-Institute, 2CICVyA - INTA Castelar, Instituto de Virología, 3Center of Molecular Immunology, Ciudad de la Habana, Cuba, 4Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology, Free University of Berlin, 5Institut of Pharmacology, Charité-University Medicine of Berlin

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Summary
Automatic Translation

This protocol describes in particular the extraction of total IgY from egg yolk by means of polyethylene glycol precipitation and gives general information about IgY technology.

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IgY Technology Chicken Antibodies Egg Yolk Polyethylene Glycol (PEG) Precipitation Immunization I.m. Vaccination Gene-Gun Plasmid-immunization Antibody-titres Boost Immunizations Laying Capacity Extraction Of Total IgY Precipitation Procedure Removal Of Lipids Dialysis IgY-extract Storage Purity Of The Extract Total IgY Per Egg Age Of The Laying Hen Total Potential Harvest Of IgY
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