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JoVE Journal Biology
Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
A High-Throughput Platform for Culture and 3D Imaging of Organoids
Journal (Biology)
A High-Throughput Platform for Culture and 3D Imaging of Organoids
Quantitation of Endothelial Cell Adhesiveness In Vitro
Journal (Biology)
Quantitation of Endothelial Cell Adhesiveness In Vitro
 

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography

Article DOI: 10.3791/3085-v • 10:10 min • July 29th, 2011
July 29th, 2011 •
1, 2, 1, 2, 1, 1
1Breakthrough Breast Cancer Research Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, 2MRC Technology

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Summary
Automatic Translation

We have developed a collagen-based in vitro assay which promotes proliferation and invasion from samples of all breast cancer subtypes. Optical Projection Tomography, a three dimensional microscopy technique was utilised to visualise and quantify tumour expansion. This assay may be used to quantify drug response of individual tumour samples.

Tags

Long-term Culture Human Breast Cancer Specimens Optical Projection Tomography Breast Cancer Mortality Western World Spread Of Breast Cancer Patient Prognosis Experimental Systems Cell Lines Primary Tumours Pleural Effusions Sub-types Of Breast Cancers Poor Prognosis Luminal B Tumours Tumour Microenvironment Culture System Rat Collagen I Cushions Histology Adipocytes Epithelial Cells Fibroblasts Malignant Epithelial Cells Squamoid Morphology Nuclear Pleomorphism Mitotic Figures Apoptotic Bodies Optical Projection Tomography (OPT) 3D Imaging Technology
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