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JoVE Journal
Immunology and Infection

This content is Open Access.

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
 

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Article DOI: 10.3791/3130-v 12:19 min July 22nd, 2011
July 22nd, 2011

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Summary

We describe a multiplex PCR for the rapid detection of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. Specific Salmonella serovars can be identified by targeting a multiplex PCR to genes and sequences unique to the O-antigen biosynthesis cluster and flagellin of a given serovar. Serovar is assigned then to a Salmonella isolate based on the appearance of specific, size amplicons (PCR product) corresponding to the target allele.

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Allelotyping PCR Identifying Salmonella Enterica Serovars Enteritidis Hadar Heidelberg Typhimurium Commercial PCR Tests Salmonella Target Genes Salmonella Serovars Public Health Significance S. Enterica Subspecies IIIa Arizona S. Enterica Subspecies I Serovar Enteritidis Salmonellosis Table Eggs Lipopolysaccharide (LPS) Flagellin (H1 And H2 Antigens) Antigenic Differences Genetic Differences Allelotyping Multiplex PCR Poultry-associated Diseases US
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