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JoVE Journal
Immunology and Infection

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分离单链DNA,双链DNA和RNA病毒使用羟基磷灰石色谱从环境社区
 
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分离单链DNA,双链DNA和RNA病毒使用羟基磷灰石色谱从环境社区

Article DOI: 10.3791/3146-v 13:47 min September 29th, 2011
September 29th, 2011

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Summary

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我们描述了独立于环境病毒社区的单链DNA,双链DNA和RNA分子的有效方法。核酸是分次使用含有磷酸盐缓冲液浓度的增加羟基磷灰石色谱。这种方法允许所有病毒从环境样品中的核酸类型的隔离。

Tags

免疫学杂志,55期,羟基磷灰石,单链DNA,双链DNA,RNA,DNA,色谱仪,病毒生态学,病毒,噬菌体
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