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Bioengineering

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JoVE Journal Bioengineering
Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
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Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart

Article DOI: 10.3791/3160-v • 09:24 min • September 13th, 2011
September 13th, 2011 •
1, 1, 1
1Department of Biomedical Engineering, Washington University in St. Louis

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This article describes the basic procedures for conducting optical mapping experiments in the Langendorff-perfused rabbit heart using the panoramic imaging system, and the dual (voltage and calcium) imaging modality.

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Multiparametric Optical Mapping Langendorff-perfused Rabbit Heart Optical Imaging Fluorescent Probes Cardiac Electrophysiology Calcium-sensitive Dyes Voltage-sensitive Dyes Transmembrane Action Potentials Calcium Transients Spatial Resolution Electrode Techniques Langendorff-perfused Rabbit Heart Model Arrhythmias Wave Patterns Ventricular Arrhythmias Excitation-contraction Coupling Rabbit Myocardium
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