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Journal / Biology / DNA Metilasyonu: bisülfit modifikasyonu ve Analiz
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DNA Metilasyonu
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DNA Metilasyonu: bisülfit modifikasyonu ve Analiz

Article DOI: 10.3791/3170-v • 12:34 min • October 21st, 2011
October 21st, 2011 •
*1, *1, 1, 1,2
1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW
* These authors contributed equally

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DNA metilasyon analizi için altın standart bisülfit dönüştürülen DNA genom dizileri. Bu yöntem, asidik koşullar altında deaminasyon bisülfit 5-methylcytosine (5-MEC) ile karşılaştırıldığında sitozin artan duyarlılık yararlanır. Unmethylated cytosines hedef genomik DNA PCR sonra metil cytosines ayırt edilebilir.

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Genetik Sayı: 56 epigenetik DNA metilasyon bisülfit 5-methylcytosine (5-MEC) PCR
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