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Journal / Biology / DNA-metylering:, dock Ändring och analys
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DNA-metylering
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DNA-metylering:, dock Ändring och analys

Article DOI: 10.3791/3170-v • 12:34 min • October 21st, 2011
October 21st, 2011 •
*1, *1, 1, 1,2
1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW
* These authors contributed equally

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Guldmyntfoten för DNA-metylering analys är genomisk sekvensering av, dock omvandlas DNA. Denna metod tar fördel av den ökade känslighet cytosin jämfört med 5-methylcytosine (5-MEC) till, dock deaminering under sura förhållanden. Unmethylated cytosines kan skiljas från metylerad cytosines efter PCR-amplifiering av målet arvsmassans DNA.

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Genetik 56 epigenetik DNA-metylering, dock (5-MEC) 5-methylcytosine PCR
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