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Biology

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Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
 

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

Article DOI: 10.3791/3187-v 08:37 min November 18th, 2011
November 18th, 2011

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Summary

Microscopic imaging of live endothelial cells expressing GFP-actin allows characterization of dynamic changes in cytoskeletal structures. Unlike techniques that use fixed specimens, this method provides a detailed assessment of temporal changes in the actin cytoskeleton in the same cells before, during, and after various physical, pharmacological, or inflammatory stimuli.

Tags

Actin Cytoskeleton Endothelial Cells GFP-Actin Permeability Adhesive Junctions Junctional Integrity Cortical Actin Belt Actin Stress Fibers Centripetal Tension Inflammatory Mediators Endothelial Permeability Live-cell Imaging Dynamic Nature Of Actin Structures
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