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Journal / Biology / Undersøgelse af Actin cytoskeleton i Live…
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Undersøgelse af Actin cytoskeleton i Live endoteliale celler, der udtrykker GFP-Actin
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Undersøgelse af Actin cytoskeleton i Live endoteliale celler, der udtrykker GFP-Actin

Article DOI: 10.3791/3187-v • 08:37 min • November 18th, 2011
November 18th, 2011 •
1, 1
1Department of Physiology, Louisiana State University Health Sciences Center

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Mikroskopisk billedbehandling af levende endothelceller udtrykker GFP-aktin tillader karakterisering af dynamiske ændringer i cytoskeletal strukturer. I modsætning til teknikker, der anvender faste prøver, giver denne metode en detaljeret vurdering af tidsmæssige ændringer i actin cytoskeleton i de samme celler før, under og efter forskellige fysiske, farmakologiske eller inflammatoriske stimuli.

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Cellebiologi endotelceller actin cytoskeleton live-cell imaging GFP lamellipodia stress fibre kymograph analyse
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