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Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

1, 2,3, 3, 2,3, 3, 4, 2,3, 2,3

1Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute at Lake Nona, 2Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 3Vanderbilt Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, 4Department of Pediatrics and Cellular and Integrative Physiology, Indiana University School of Medicine

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    The hyperinsulinemic-euglycemic clamp, or insulin clamp, is the gold standard for assessing insulin action in vivo. A method for performing insulin clamps in mice is described. This includes a method for arterial catheterization that permits experiments to be performed in conscious, unrestrained mice with minimal stress.

    Date Published: 11/16/2011, Issue 57; doi: 10.3791/3188

    Cite this Article

    Ayala, J. E., Bracy, D. P., Malabanan, C., James, F. D., Ansari, T., Fueger, P. T., et al. Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice. J. Vis. Exp. (57), e3188, doi:10.3791/3188 (2011).


    Type 2 diabetes is characterized by a defect in insulin action. The hyperinsulinemic-euglycemic clamp, or insulin clamp, is widely considered the "gold standard" method for assessing insulin action in vivo. During an insulin clamp, hyperinsulinemia is achieved by a constant insulin infusion. Euglycemia is maintained via a concomitant glucose infusion at a variable rate. This variable glucose infusion rate (GIR) is determined by measuring blood glucose at brief intervals throughout the experiment and adjusting the GIR accordingly. The GIR is indicative of whole-body insulin action, as mice with enhanced insulin action require a greater GIR. The insulin clamp can incorporate administration of isotopic 2[14C]deoxyglucose to assess tissue-specific glucose uptake and [3-3H]glucose to assess the ability of insulin to suppress the rate of endogenous glucose appearance (endoRa), a marker of hepatic glucose production, and to stimulate the rate of whole-body glucose disappearance (Rd).

    The miniaturization of the insulin clamp for use in genetic mouse models of metabolic disease has led to significant advances in diabetes research. Methods for performing insulin clamps vary between laboratories. It is important to note that the manner in which an insulin clamp is performed can significantly affect the results obtained. We have published a comprehensive assessment of different approaches to performing insulin clamps in conscious mice1 as well as an evaluation of the metabolic response of four commonly used inbred mouse strains using various clamp techniques2. Here we present a protocol for performing insulin clamps on conscious, unrestrained mice developed by the Vanderbilt Mouse Metabolic Phenotyping Center (MMPC; URL: This includes a description of the method for implanting catheters used during the insulin clamp. The protocol employed by the Vanderbilt MMPC utilizes a unique two-catheter system3. One catheter is inserted into the jugular vein for infusions. A second catheter is inserted into the carotid artery, which allows for blood sampling without the need to restrain or handle the mouse. This technique provides a significant advantage to the most common method for obtaining blood samples during insulin clamps which is to sample from the severed tip of the tail. Unlike this latter method, sampling from an arterial catheter is not stressful to the mouse1. We also describe methods for using isotopic tracer infusions to assess tissue-specific insulin action. We also provide guidelines for the appropriate presentation of results obtained from insulin clamps.


    1. Preparation of catheters and Mouse Antenna for Sampling Access (MASAtm)

    1. Prepare arterial catheter by inserting a 1.3 cm piece of PE-10 (0.011 inch inner diameter) into a 6 cm piece of silastic tubing (0.012 inch inner diameter) as shown in Figure 1A. Bevel the tip of the PE-10 with a scalpel so the length from the end of the silastic to the tip of the bevel is 0.9 cm.
    2. Prepare venous catheter by sliding a 1 mm piece of silastic tubing (0.020 inch inner diameter) 1.1 cm from the beveled end of a 6 cm piece of silastic tubing (0.012 inch inner diameter) as shown in Figure 1B. The 1 mm piece of silastic is used as a restraining bead.
    3. To prepare a MASAtm, insert each of two 1.3 cm pieces of 25 gauge stainless steel connectors into each of two 3 cm pieces of PE-20 (0.015 inch inner diameter).
    4. Secure the PE-20/connectors to each other by sliding a 5 mm piece of silastic tubing (0.040 inch inner diameter) over the area where the steel tubes and PE-20 meet.
    5. Bend the steel tubes to a ˜120° angle and separate each tube by ˜ 45°.
    6. Place completed rig in a dollop of medical silicone adhesive such that the PE-20 tubing is vertical and the ends of the stainless steel tubes extend beyond the adhesive (Figure 1C). Allow this to set for 24h.

    2. Surgical catheterization

    1. Prior to surgery, sterilize catheters with 70% ethanol, fill them with heparinized saline (200 U heparin/ml saline) and insert stainless steel plugs.
    2. Anesthetize mouse, preferably using a method that continuously delivers the anesthetic agent (e.g. inhaled isoflurane).
    3. Using sterile technique, remove hair from the incision sites using clippers and/or a depilatory cream and disinfect the skin with alcohol followed by a betadine scrub. For catheter insertion, remove hair on the region extending from the lower jaw to the top of the rib cage and between the clavicles. For externalization of the catheters behind the head, remove hair in the area between the base of the skull and the interscapular region and disinfect the skin with alcohol followed by a betadine scrub.
    4. Lay the mouse on its back on a warming surface and under the viewing area of a surgical microscope. Secure the tail and extremities with surgical tape. Secure the head in the nose cone delivering the anesthesia.
    5. Make a small vertical midline incision 5 mm cephalic to the sternum. Using forceps, blunt dissect the tissue to expose the left sternomastoid muscle. Reflect this muscle to expose the left carotid artery. Gently tease off connective tissue from the artery. It is important at this point to isolate the vagus nerve from the artery without damaging either the artery or the nerve.
    6. Isolate the artery and ligate the cephalic end with silk suture. Loosely knot another piece of suture on the caudal end of the exposed vessel.
    7. Clamp vessel with a micro-serrefine clamp on the caudal end and cut just below the ligated end with spring scissors. Carefully insert catheter as far as the clamp. Carefully release the micro-serrefine clamp and advance the catheter to the silastic-PE junction.
    8. Tie both ligatures securely to the catheter and confirm that the catheter samples by connecting the free end of the catheter to a sampling syringe.
    9. Make another incision 5 mm to the right of midline and about 2 mm caudal to the first incision. Using forceps, blunt dissect the tissue to expose and isolate the right jugular vein.
    10. Carefully ligate the cephalic end with silk suture and loosely tie another piece of suture at the caudal end.
    11. Cut just below the cephalic ligature with spring scissors and insert the catheter up to the restraining bead. Tie a suture behind the bead and confirm that the catheter samples.
    12. Turn the mouse over and make a small incision between the shoulder blades.
    13. Tunnel a 14-gauge needle under the skin from the incision for the arterial catheter, on the front of the mouse, to the interscapular incision on the back. Thread the arterial catheter through the needle to exteriorize it at the back of the mouse. Repeat this for the jugular vein catheter by tunneling the 14-gauge needle under the skin on the right side of the mouse from the incision site on the front to the interscapular incision on the back.
    14. Clamp the arterial catheter with a micro-serrefine clamp at the incision site between the shoulder blades. Cut the catheter ˜1 cm above this clamp. Place the MASAtm with the stainless steel connectors facing towards the head of the mouse. Connect the arterial catheter to the stainless steel connector pointed towards the left side of the mouse. Take care to ensure there are no holes or kinks in the catheter. Repeat for the venous catheter, connecting it to the stainless steel connector pointing to the right side of the mouse.
    15. Insert the MASAtm into the incision between the shoulder blades. The PE-20 tubing corresponding to the jugular vein catheter should be to the right side of the mouse and the PE-20 tubing corresponding to the arterial catheter should be to the left.
    16. Close ventral and dorsal incisions with nylon suture. For the dorsal closing, the suture can be run through the hardened silicone of the MASAtm to secure it in place. Confirm patency of the catheters using a flushing solution containing heparinized saline and an antibiotic to minimize the risk of infection. Place mouse in a warmed, clean cage for immediate recovery. Figure 2 shows the finished product.
    17. Allow the mouse to recover for at least 5 days. Monitor weight and overall health. Utilize the appropriate post-operative analgesic regimen as approved by the institution's Animal Care and Use Committee.

    3. Hyperinsulinemic-euglycemic clamp

    1. Fast the mouse for 5-6h. As a reference, the time t = 0 min refers to the end of the fast and the beginning of the insulin and glucose infusion (i.e. the clamp period).
    2. The setup and time line for a typical experiment are shown in Figure 3. Use Micro-Renathane or equivalent tubing for infusion and sampling lines. Suspend a dual-channel swivel above the mouse. This serves as a hub between the mouse and infusion/sampling syringes. During the experiment, the mouse remains in a home cage or similar container and is tethered to the swivel.
    3. Prior to connecting the mouse, fill the arterial sampling line with heparinized saline (10 U heparin/ml saline) and place a stainless steel connector at the bottom end of the line. Leave a syringe with heparinized saline (clearing syringe) connected to the top of the sampling line. This will be used to draw up blood samples.
    4. Fill the venous infusion line with non-heparinized saline starting from the infusion port of the swivel (Segment A in Figure 3A) all the way to the bottom of the line. Plug the top end of the line and place a stainless steel connector (or a Y-connector if a bolus is to be administered) at the bottom end of the line. If an isotopic glucose tracer (e.g. [3-3H]glucose) is being infused, secure a 1 ml syringe containing the tracer to an infusion syringe. Fill the venous infusion line with tracer instead of saline (Figure 3B).
    5. Three hours into the fast, weigh the mouse and connect the PE-20 for the jugular vein and arterial catheters to the infusion and sampling lines, respectively.
    6. If administering [3-3H]glucose tracer, begin a primed-continuous tracer infusion at t = -90 min (Figure 3C). A typical priming dose is 1 μCi. Prepare a 0.05 μCi/μl [3-3H]glucose solution in non-heparinized saline. Load the solution into a 1 ml syringe and secure the syringe in an infusion pump. Administer the priming dose by infusing 20 μl/min for 1 min. Follow with a continuous infusion of 0.05 μCi/min (1 μl/min) for a 90 min equilibration period.
    7. Prepare infusates of insulin and glucose. Insulin is prepared in non-heparinized saline containing 3% plasma as a carrier (an appropriate concentration of BSA can also be used). Glucose infusates are commercially available in a variety of concentrations (5, 20 and 50%).
    8. Prepare saline-washed erythorocyte infusate by obtaining whole blood from a donor mouse, preferably of the same strain background as the experimental mouse. Typically 1 ml of whole blood is needed per study mouse. Centrifuge blood to separate erythrocytes. Wash erythrocytes with 10 U/ml heparinized saline and centrifuge to discard the saline. Determine the volume of erythrocytes and resuspend in an equal volume of 10 U/ml heparinized saline.
    9. Draw each infusate into a 1 ml syringe and secure each syringe to an individual infusion pump. Connect each syringe to a 4-way connector (Figure 3).
    10. At t = -15 min take a blood sample by slowly drawing up 50-100 μl of blood into the clearing syringe. Clamp the arterial sampling line and remove the clearing syringe. Using a hand-held glucose meter, take a blood glucose reading by removing the clamp on the arterial sampling line and allowing blood to flow into the glucose meter strip.
    11. Once the glucose measurement is taken, clamp the arterial sampling line and insert a blunt-needle syringe (sampling syringe) into the arterial sampling line. Remove the clamp and draw a volume of blood (see Note) into the sampling syringe. Clamp the arterial sampling line and remove the sampling syringe. Insert the clearing syringe back into the arterial sampling line. Draw up on the plunger to remove any air bubbles and re-infuse the 50-100 μl of blood that was originally drawn.

    Note: The volume of blood sampled depends on the analysis being performed. For example, analysis of [3-3H]glucose concentration requires 10 μl of plasma, so 50 μl of blood are drawn. This yields 20-30 μl of plasma, which is sufficient for the analysis plus additional plasma if needed. Measurements of hormones and other metabolites (e.g. insulin, free fatty acids) require sampling of additional blood.

    1. Dispense the blood in the sampling syringe into an EDTA-coated microtube. Centrifuge and collect the plasma. Keep the plasma on ice until the end of the study or immediately store at -20°C.
    2. Repeat steps 3.10 through 3.12 at t = -5 min. Obtain additional blood (50 μl) for measurement of baseline plasma insulin levels. Measure baseline hematocrit by drawing blood into a heparin- or EDTA-treated capillary tube. The measurements obtained from plasma samples at t = -15 and -5 min represent baseline (i.e. fasting) values.
    3. After the sample at t = -5 min, fill the infusion lines for glucose, insulin and saline-washed erythrocytes up to the 4-way connector. Connect the 4-way connector to the tubing attached to the swivel infusion port (or the tubing connected to the Y-connector if infusing [3-3H]glucose) as shown in Figure 3.
    4. Begin infusing the saline-washed erythrocytes first. Set the rate of infusion to replace the total volume of blood being sampled over the duration of the study (e.g. if a total of 500 μl of blood are sampled over 120 min of the study, set the infusion rate to 4.2 μl/min). In contrast to the other infusates, the erythrocyte solution is red. Infusing this solution first allows for any potential resistance or obstructions in the infusion lines to be identified and corrected.
    5. Once the erythrocyte infusate reaches the mouse, begin the insulin and glucose infusions. This is now t = 0 min. Insulin is infused at a constant, pre-determined rate. An insulin infusion rate of 4 mU•kg-1•min-1 will typically suppress endogenous glucose production by 80-100% and stimulate glucose disappearance by 2-3 fold. The initial glucose infusion rate (GIR) is estimated based on the baseline blood glucose levels and previous experience.
    6. If infusing [3-3H]glucose, one may elect to increase the tracer infusion rate to match the estimated increase in glucose turnover (typically a 2-3 fold increase).
    7. Considering the high rate of glucose turnover in the mouse, blood samples should be obtained from the arterial line no less than every 10 min for the measurement of glucose concentration over the duration of the experiment. Adjust the GIR to achieve and maintain target euglycemia (Figure 3C). This target can vary depending on the model or the aims of the study. A good target glucose concentration is 150 mg•dL-1 since this is a typical 6h fasted glucose level for a chow-fed C57Bl/6J mouse.
    8. The goal is to achieve euglycemia quickly, ideally within the first 40-50 min, and to have glucose and GIR stable by the beginning of the steady-state period (t = 80 min).
    9. If infusing [3-3H]glucose, obtain additional blood at t = 80, 90, 100, 110 and 120 min for the measurement of plasma [3-3H]glucose specific activity.
    10. Collect additional blood at t = 100 and 120 min for measurement of plasma insulin and any other hormone(s) or metabolite(s). At t = 110 min, draw blood into a heparin- or EDTA-treated capillary tube for measurement of clamp hematocrit.
    11. After the sample at t = 120 min is taken, 2[14C]deoxyglucose can be administered for the measurement of tissue-specific glucose uptake. Administer a 12 μCi bolus into the bolus line connected to the jugular sampling line (Figure 3B).
    12. Obtain blood samples (50 μl) from the arterial sampling line at t = 2, 15, 25 and 35 min after administration of the bolus for the measurement of plasma 2[14C]deoxyglucose levels.
    13. After the last sample, anesthetize the mouse with an infusion of pentobarbital given directly into the arterial line. Quickly dissect any tissues required for the assessment of glucose uptake (e.g. skeletal muscles of various types, adipose tissue, heart, brain) and any other tissues (e.g. liver, spleen, kidneys). Snap freeze tissues in liquid nitrogen and store at -80°C until analyzed. Tissue glucose is analyzed by measuring the accumulation of phosphorylated 2[14C]deoxyglucose in the frozen tissues and the disappearance of 2[14C]deoxyglucose from the plasma.

    4. Representative Results

    An example of results obtained from an insulin clamp experiment is shown in Figure 4. This example shows the ability of a high fat diet to precipitate insulin resistance in mice. All presentations of insulin clamp results must include the following to be interpretable: a time course of blood glucose levels, a time course of the GIR and plasma insulin levels (baseline and clamp). As shown here, fasting glucose (Figure 4A) and insulin (Figure 4C) levels are higher in mice fed a high fat diet, indicative of insulin resistance. Presenting a time course of glucose levels throughout the clamp study (Figure 4A) allows the reader to assess how well euglycemia was maintained, which is indicative of the quality of the clamp. Similarly, a time course of the GIR (Figure 4B) allows the reader to determine how quickly a steady-state was achieved. Showing these data as time courses is significantly more informative than the conventional practice in the mouse insulin clamp literature of presenting a 2-hour experiment as a single datum point representing average values from an undefined "clamp" period (4-13). In the present example, glucose levels were equal between the control and high fat-fed groups, but the GIR was significantly lower in the high fat-fed group (Figure 4B). This is indicative of an impairment in whole-body insulin action. Clamp insulin levels were also higher in the high fat-fed group (Figure 4C), further supporting the presence of an insulin resistant phenotype in these mice. The use of isotopic tracer infusions allows for the assessment of insulin action in specific tissues. [3-3H]glucose is used to estimate the rate of endogenous glucose appearance (endoRa), which is an index of hepatic glucose production (HGP) and the rate of whole-body glucose disappearance (Rd). Whereas insulin completely suppresses HGP in control mice, this is impaired in mice fed a high fat diet (Figure 4D). Similarly, the ability of insulin to stimulate Rd in control mice is compromised in mice fed a high fat diet (Figure 4E). 2[14C]deoxyglucose is used to assess the glucose metabolic index (Rg), a measure of tissue-specific glucose uptake. As seen in this example, insulin-stimulated glucose uptake into skeletal muscle is impaired in mice fed a high fat diet (Figure 4F).

    Figure 1
    Figure 1: Preparation of arterial (A) and (B) venous catheters and (C) MASAtm. Arterial catheters are prepared by inserting a 1.3 cm piece of PE-10 about 3 mm into a 6 cm piece of 0.012"ID silastic. The PE-10 tip is beveled such that the length from the bevel to the silastic is 0.9 cm. Venous catheters are made by sliding a small piece of 0.020"ID silastic 1.1 cm from the beveled end of a 6 cm piece of 0.012"ID silastic. The 0.020"ID silastic piece acts as a restraining bead to secure the catheter to the jugular vein. For assembly of the MASAtm, each of two 1.3 cm 25-gauge connectors is inserted into each of two 3 cm pieces of PE-20. These are held together by a small piece of 0.040"ID silastic. The connectors are bent to a 120° angle and separated at a 45° angle. The entire assembly is immersed in medical silicone adhesive.

    Figure 2
    Figure 2: Catheterized mouse. Catheters are surgically implanted in the left carotid artery and the right jugular vein. The free ends of the catheters are externalized behind the head and connected to a MASAtm. The MASAtm is inserted subcutaneously between the shoulder blades. This allows for vascular access during insulin clamp experiments without the need to restrain, handle or anesthetize the mouse.

    Figure 3
    Figure 3: Depiction of the setup and time line for an insulin clamp experiment. The mouse is tethered to a dual-channel swivel that acts as a hub for infusion and sampling syringes. Typical setups for experiments not using tracer infusions (A) and using both [3-3H]glucose and 2[14C]deoxyglucose (B) are shown. A time line of procedures for setting up and performing the insulin clamp (C) is also shown. During the clamp, blood samples (blood) are taken every 10 min to measure blood glucose. The GIR is adjusted accordingly to maintain euglycemia. Samples for baseline blood glucose, plasma insulin, and plasma [3-3H]glucose are taken at t = -15 and -5 min. Samples for clamp plasma [3-3H]glucose are taken at t = 80, 90, 100, 110 and 120 and for clamp insulin at t = 100 and 120 min. 2[14C]deoxyglucose is administered after the sample at t =120 min and blood is collected at t = 2, 15, 25 and 35 min after. Tissues are taken after the t = 35 min sample.

    Figure 4
    Figure 4: Results from an insulin clamp experiment comparing mice on a control diet (Chow) to mice on a high fat diet (HFD). Time course of arterial glucose (A) and GIR (B), baseline and clamp insulin (C), EndoRa (D), and Rd (E) and skeletal muscle (gastrocnemius and vastus lateralis) Rg (F) are shown. All results indicate the effect of high fat feeding to induce insulin resistance.


    The hyperinsulinemic-euglycemic clamp, or insulin clamp, is widely considered the "gold standard" method for assessing insulin action in vivo. This technique has been applied to several species including humans, dogs, rats and mice. Given the growing number of transgenic mouse models for metabolic disorders, the miniaturization of the technique for use in the mouse has provided significant advances to metabolic research.

    While the concepts behind the insulin clamp are straightforward, in practice there are different approaches for performing insulin clamp experiments. This is not a trivial point, since the manner in which the experiment is performed affects results obtained1. Here we present the protocol used by the Vanderbilt MMPC. The key distinction between our protocol and that of others is that we use an arterial catheter for obtaining blood samples. This is in contrast to the more widely used approach of obtaining blood samples by severing the tip of the tail4, 7, 11, 12, 14-17. The advantage of sampling from an arterial catheter is that the experiment is conducted in a conscious and unrestrained mouse. Sampling from the tail often requires restraint and increases indices of stress when large blood samples are acquired1. Stress hormones stimulate endogenous glucose production and impair glucose disposal18, 19, potentially giving the appearance of an insulin resistant phenotype. Sampling from the severed tail may require special Institutional Animal Care and Use Committee approval because of its stressful nature. The arterial catheterization procedure was developed to avoid the stress to the mouse of severing the tail.

    A key aspect of performing insulin clamps is the ability to maintain euglycemia. There are no algorithms that can correctly predict how the GIR should be adjusted based on blood glucose readings. Like the surgery, personnel conducting insulin clamp experiments will become proficient in maintaining reasonable euglycemia only through experience. It is important to note that because of their higher metabolic rate the data obtained from mouse studies will be inherently noisy. This makes the complete presentation of data, including time courses of glucose and GIR and absolute values for plasma insulin, endoRa, Rd and Rg crucial for the ability of any reader to interpret results. The high glucose flux rates in the mouse (approximately 5 times higher than the rate in humans) warrant a high frequency of glucose sampling. While the blood volume of the mouse is limited, a minimum sampling frequency of once every 10 minutes is necessary to be certain that an adequate clamp has been achieved.

    As shown in Figure 4, clamp insulin levels can be different between groups. Factors such as diet interventions, transgenic manipulations or differences in background strains can affect fasting insulin levels, which can subsequently affect clamp insulin levels. Interpreting results when clamp insulin levels are different can be problematic. This can be experimentally addressed by performing pilot experiments to select insulin infusion rates that achieve equivalent clamp insulin levels between groups. Alternatively, somatostatin can be used to inhibit pancreatic hormone secretion, and insulin and glucagon can be replaced at experimentally controlled rates. This latter approach is more commonly done in insulin clamps on rats than mice. If these experimental approaches are not taken, the steady-state GIR can be normalized to the clamp insulin level, or an insulin sensitivity index (SI) can be derived from clamp data as SI=GIR/(G•ΔI), where G is the steady-state glucose concentration and ΔI is the difference between fasting and clamp insulin concentrations. One assumption with either approach is that the clamp insulin level achieved is within the range where insulin sensitivity is linearly related to the insulin level according to the group being studied. This latter assumption may not apply when comparing insulin resistant and insulin sensitive groups. Ideally, an insulin dose response curve should be generated to select the appropriate insulin infusion. However, because of the requirement for additional experiments, this is rarely done.

    The versatility provided by arterial catheterization extends to experimental approaches beyond euglycemic clamps. For example, hyperglycemic clamps, in which glucose is infused at a variable rate to maintain hyperglycemia relative to fasting glucose, can be used to assess endogenous pancreatic function in vivo2, 20, 21. The measurement of first phase insulin secretion during this test requires frequent acquisition of blood samples (i.e every 2-5 min), which is not feasible when obtaining samples from the tip of the tail. Furthermore, elevated catecholamines resulting from tail sampling can impair insulin secretion and enhance glucagon secretion22. The insulin clamp protocol can also be modified to allow for glucose levels to fall to relative hypoglycemia to assess counter-regulatory response2, 23, 24. Arterial catheterization can also be used to assess the dynamics of glucose metabolism during exercise25-30. This is significantly advantageous over conventional approaches conducted at single time points pre- and post-exercise or in isolated muscles ex vivo. The techniques presented here can also be used to assess not just glucose, but also fatty acid metabolism31.


    No conflicts of interest declared.


    This work was supported by Grant 5-U24-DK059637-10 to the Vanderbilt Mouse Metabolic Phenotyping Center.


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    Dear auther
    I would like to know if the disc accomodating the arterial and venus cathters tunneled under the skin and allowing the catheter antenas to protude is a commercial item and if not how do you produce it ?
    Thank you Rona

    Posted by: Rona S.November 29, 2012, 8:09 AM

    Dear Rona,
    If you are referring to the MASA, please refer to section 1 of this article - "Preparation of catheters and the Mouse Antenna for Sampling Access". This section describes how to make the disc. Bend two 1.3 cm pieces of ²5 gauge steel tubing to about 1²0 degrees. Insert a 3 cm piece of PE-²0 into each steel tube. Secure both pieces of steel tubing to each other with a 5mm piece of silastic tubing. Dip the steel tubes into a drop of medical grade silicone adhesive such that the PE-²0 is vertical and the free steel tube ends come out of the bottom of the drop at about a 45 degree angle separation. Let the adhesive become solid overnight. I hope this helps.
    Best regards,
    Julio Ayala

    Posted by: Julio A.November 29, 2012, 8:46 AM

    Dear Julio
    Yes this was my question
    Thank you for the explenation
    All the best Rona

    Posted by: Rona S.November 29, 2012, 9:39 AM

    Hello Julio, I wonder how you maintain catheters. Should I flush them with heparinized saline everyday? Even if flushing everyday, catheters are clogged easily. Do I need higher heparin than 200 U/ml for flushing? Give me some advice, please? Thank you,

    Posted by: Teayoun K.March 26, 2014, 1:17 PM

    Hello Teayoun,
    This may depend on the material that you use for the tubing that is attached to the MASA. Originally, we used microrenathane tubing and would flush the lines daily with 200 U/ml hep saline. We have since started using PE-20 instead of microrenathane and find that we do not need to flush the lines (sometimes we will flush the lines 3 days after surgery just for good measure). With either approach, you will inevitably have some catheters clog, but this should not occur often. If you are flushing your lines daily and most of your catheters are still clogging, then there is likely another issue (perhaps the placement of the catheter is not correct?). Is this the arterial or the venous catheter? If it is the arterial catheter, is it sampling on the day of the surgery? How long until it clogs?


    Posted by: Julio A.March 26, 2014, 2:06 PM


    Thank you for quick response. Yes, I use microrenathane to make venous catheter body, but the tip of catheter is PE-20. If the material itself is problem, I will try PE-20 to make the body part of catheter. The catheter was working well (infusion was easy and smooth as a confirmation after implantation surgery done). Arterial catheter is a commercial one from Alzet (designed for mouse jugular vein, but works well for mouse carotid artery). I've heard some comments from others. heparin causes lipolysis and changing body metabolism and streptokinase may be a problem due to microbial material causing immune response. I'm still not 100% comfortable on catheter maintenance. Metal plug was used, but it's same. I don't know exact reason yet. I wish I could figure it out soon. Thank you,

    Posted by: Teayoun K.March 28, 2014, 12:20 PM

    I would not recommend using PE or microrenathane for the catheter body (either vein or artery). PE is too rigid and microrenathane is too porous and prone to react (clot). It is my understanding that the Alzet catheters are made with polyurethane which, like the microrenathane, is too porous. I woudl recommend making the catheters as shown in this article (silastic for the vein and silastic with a PE-10 tip for the artery). Heparin will have an effect on lipolysis, but the amounts and frequency with which you flush should not have significant effects on metabolism. Also, when checking the catheters after the surgery, make sure that you can draw blood, not just infuse smoothly. Sometimes you can infuse very smoothly but cannot draw blood, which means that the catheter is not in place.

    Posted by: Julio A.March 28, 2014, 3:42 PM

    Dear author,
    Can you please provide a product number and purchasing source for the "medical silicone adhesive" used for MASA construction?
    thank you
    Joe C.

    Posted by: Joseph C.April 8, 2014, 5:11 PM

    Hi Joe C.

    You can get it from Fisher:

    FACTOR II INC MEDICAL ADHESIVE SIL TYPE A Catalog No.: NC9938868 Medical Adhesive, Type A

    Good luck!

    Posted by: Julio A.April 11, 2014, 4:15 PM

    Dear Dr.Ayala,

    We performed surgery following your protocol, 5 days later, animals gain body weight back and both catheters stay open.We try to clamp the blood glucose level around 150mg/dl, The insulin dose is 4mU/kg.min. The fasting glucose level of our mice is around 120, During the H3 tracer infusion phase, the blood glucose level normally dropped to around 100, but will not fluctuate a lot. However when we stared insulin and glucose infusion, the blood glucose can be stable for 40 mins then suddenly drop to below 100. Then we increased GIR, the blood glucose will come back to 150s but will not stay there, after 30 mins to 40 mins it will dip again.
    We dissect the mice after surgery, to make sure there was no leakage.
    We paid attention to small blood sample size,donor blood,and we controlled temperature, we kept environment quiet. We fast mice 3hrs before clamp. Could you please give us some suggestions for trouble shooting? Thanks a lot.

    Posted by: Kai M.April 15, 2014, 11:17 AM

    Without seeing our data, particularly the time course of your glucose infusion and glucose levels, I really cannot tell you whether your experience is unusual. When you say that "it dips again", does it dip below 100 mg/dL? Does it dip by 5, 10 mg/dL? How much are you changing the glucose infusion rate? Are these wild-type mice or a transgenic line? You can contact me directly at if you want to discuss further.

    Posted by: Julio A.April 16, 2014, 10:32 AM

    Dear Dr.Ayala,

    I used to use micro-renathane tubing to make venous catheter tip, however Micro-renathane is rigid, half of my catheter penetrate the venous wall, I end up with all my infusate leaked into chest cavity. Then I start to follow your instruction to make venous catheter with silastic tubing. However, I found quite a few my surgery failed because my ligation on the catheter will leak after 3,4 days. No matter how tight I made the ties, or add 1 or two more sutures, it didn't improve. I always found a big lump of infusate formed after clamp subcutaneously near the neck. I am using 6-o silk suture now. Can you please give me some hint to improve the procedure?


    Posted by: Kai M.October 2, 2014, 12:32 PM

    Hello Kai. I am Carlo and the hands doing the surgical procedure parts of the video are attached to my body :-). Kidding aside, do the opposite. Try tying over the vessel/catheter with the least possible pressure. The guide is: once you see that the suture knot is deforming the silastic tubing, you are tying too tight! You only need the suture to be snug and it should hold in the vessel without affecting the patency or causing it to leak. Let me know if this tip works.

    Posted by: Carlo M.October 2, 2014, 1:29 PM

    Thanks. I will try it!


    Posted by: Kai M.October 2, 2014, 4:11 PM

    Dear Kai,
    Are you making the silastic "collar" using 0.020" ID silastic as shown in Fig. 1B? If you tie your suture behind this collar, it should secure the catheter in the vessel.

    Posted by: Julio A.October 2, 2014, 1:05 PM

    Dr.Ayala, Thanks for your reply, Yes I do, the leakage always happens around the ties before the docking beads. The ties can secure the catheter for about 4days. I doubt if my 7-o suture is too sharp, ( I typo a 6-O suture in the previous comment).so the it damage the wall of blood vessel . Can you tell me what size do you use? Another concern is if the silastic tubing is too soft. The leakage rarely happens when I use Micro-renathane tubing. Thanks.

    Posted by: Kai M.October 2, 2014, 1:25 PM

    Also, just out of curiosity, are you making your venous catheter as a single 6cm long piece of silastic? I ask because in your original comment, you referred to the "venous catheter tip". I was just wondering if you were trying to make it the same as the arterial catheter. It should just be a single piece of silastic with the collar slipped over it. I just saw that Carlo Malabanan posted a reply as well. He is an expert at this surgery.

    Posted by: Julio A.October 2, 2014, 1:38 PM

    We use 7-0 sutures and silastic tubing and have not had leaking problems with the silastic. I will confer with my colleagues at Vanderbilt to see if they have seen this before.

    Posted by: Julio A.October 2, 2014, 1:31 PM

    Dear Malabanan

    Could you kindly explain why 7-o is better than 6?


    Posted by: Kai M.October 2, 2014, 2:56 PM


    I called the part in fornt of docking beads the "tip" , yes I used a whole piece of silastic tubing as my catheter.Thanks.

    Posted by: Kai M.October 2, 2014, 1:47 PM

    Dr.Ayala and Dr.Malabanan
    I figured out the reason for venous leakage is didn't insert the catheter deep enough, since I worry about the tip of venous catheter penetrating the venous wall, I only use 9mm long tip. Which cause a dead space in the vein where blood will stay and form clot, once the clot block the vein, the pressure will build up and eventually burst the venous wall when I start to infuse.Now as you suggested I am using 11mm long tip, which will stay close enough to the heart, and no clot will form before the opening of catheter. Thanks For your help.

    Posted by: Kai M.November 4, 2014, 1:04 PM

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