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La purificación de Hsp104, una proteína Disaggregase
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La purificación de Hsp104, una proteína Disaggregase

Article DOI: 10.3791/3190-v • 07:18 min • September 30th, 2011
September 30th, 2011 •
1, 1, 1
1Department of Biochemistry and Biophysics, University of Pennsylvania

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Aquí se describe un protocolo para la purificación de gran actividad Hsp104, un AAA hexameric + proteína de la levadura, que se acopla la hidrólisis de ATP con el desglose de proteínas. Este esquema se aprovecha de una construcción de His6-etiquetados para la purificación de afinidad de

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Biología Molecular Número 55 Neurociencia Hsp104 AAA + disaggregase choque térmico el amiloide priones
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