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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
Journal (Immunology and Infection)
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Journal (Immunology and Infection)
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
 

Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions

Article DOI: 10.3791/3261-v • 08:13 min • September 2nd, 2011
September 2nd, 2011 •
1, 1
1Division of Basic Medical Sciences, Mercer University School of Medicine

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Summary
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This method allows characterization of extended bacterial co-culture with EpiAirways, primary human respiratory epithelial tissue grown at the air-liquid interface, a biologically relevant in vitro model. The approach can be used with any microbe that is amenable to long-term co-culture.

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EpiAirway Model Host-pathogen Interactions Nontypeable Haemophilus Influenzae Respiratory Mucosa Bacteria Human Cells Long-term Co-culture Primary Human Respiratory Epithelial Tissues Air-liquid Interface Tight Junctions Ciliated Cells Nonciliated Cells Goblet Cells Mucin Production Cytokine Profile Bacterial Infections
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