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Journal / Immunology and Infection / Colorectal Cancer Cell Surface Protein profilage…
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Immunology and Infection
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Colorectal Cancer Cell Surface Protein profilage utilisant une biopuces d'anticorps et de multiplexage par fluorescence
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Colorectal Cancer Cell Surface Protein profilage utilisant une biopuces d'anticorps et de multiplexage par fluorescence

Article DOI: 10.3791/3322-v • 15:18 min • September 25th, 2011
September 25th, 2011 •
1, 1, 2, 3, 4, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital

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Nous avons décrit une procédure pour la désagrégation du cancer colorectal (CCR) pour produire des cellules viables unique, qui sont ensuite saisies sur biopuces d'anticorps reconnaissant les antigènes de surface sur mesure (CRC DotScan micropuce). Les sous-populations de cellules lié à la puce peut être profilée par multiplexage de fluorescence à l'aide d'anticorps monoclonaux marqués avec des colorants fluorescents.

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Immunologie numéro 55 le cancer colorectal les leucocytes biopuces d'anticorps le multiplexage la fluorescence des antigènes CD,
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