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JoVE Journal Neuroscience
Patch-clamp Kapacitans Målinger og Ca 2 + Imaging på Single nerveterminaler i Retinal Skiver
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Patch-clamp Kapacitans Målinger og Ca 2 + Imaging på Single nerveterminaler i Retinal Skiver

Article DOI: 10.3791/3345-v • 09:17 min • January 19th, 2012
January 19th, 2012 •
1, 1, 1
1The Vollum Institute, Oregon Health and Science University

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Her beskriver vi en protokol for forberedelse af agar-embedded retinal skiver, der er egnet til elektrofysiologi og Ca2 + billeddannelse. Denne metode gør det muligt at studere bånd-typen synapser i retina mikrokredsløb med direkte patch-clamp optagelser af en enkelt præsynaptiske nerveender.

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Neurovidenskab synaptisk fysiologi Axon terminal synaptisk bånd nethinden mikrokredsløb guldfisk zebrafisk hjerne skiver patch-clamp membran kapacitans målinger calcium-imaging exocytose transmitter frigivelse
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