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JoVE Journal
Immunology and Infection

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In vitro Uncoating af HIV-1 kerner
 
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In vitro Uncoating af HIV-1 kerner

Article DOI: 10.3791/3384-v 10:49 min November 8th, 2011
November 8th, 2011

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Summary

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Click here for the English version.

Uncoating er et væsentligt skridt i den tidlige fase af HIV-1 livscyklus og er defineret som demonteringen af ​​kapsid skallen og frigivelsen af ​​den virale ribonucleoprotein kompleks (vRNP). Her vil vi demonstrere teknikker til at isolere intakte kerner fra HIV-1 virioner og til at kvantificere deres uncoating

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Immunologi Lentivirus hiv virus infektion kapsid 293T Celler
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