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Journal / Immunology and Infection / In vitro uncoating van HIV-1 Kernen
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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro uncoating van HIV-1 Kernen
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In vitro uncoating van HIV-1 Kernen

Article DOI: 10.3791/3384-v • 10:49 min • November 8th, 2011
November 8th, 2011 •
1, 1
1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine

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Uncoating is een essentiële stap in de vroege fase van de HIV-1 levenscyclus en wordt gedefinieerd als de demontage van het capside shell en de vrijlating van de virale ribonucleoproteïne complex (vRNP). Hier tonen we technieken voor het isoleren van intacte kernen van HIV-1 virionen en voor de kwantificering van hun uncoating

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Immunologie lentivirus HIV virus infectie capside 293T-cellen
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