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Journal / Immunology and Infection / In vitro Uncoating von HIV-1-Kerne
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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro Uncoating von HIV-1-Kerne
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In vitro Uncoating von HIV-1-Kerne

Article DOI: 10.3791/3384-v • 10:49 min • November 8th, 2011
November 8th, 2011 •
1, 1
1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine

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Uncoating ist ein wesentlicher Schritt in der frühen Phase der HIV-1 Lebenszyklus und wird als der Demontage der Kapsid-Shell und die Freisetzung des viralen Ribonukleoproteinkomplex (vRNP) definiert. Hier zeigen wir Techniken zur Isolierung von intakten Kernen aus HIV-1 Virionen und zur Quantifizierung ihrer uncoating

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Immunologie Lentivirus HIV Virus Infektion Kapsid 293T-Zellen
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