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Journal / Bioengineering / Tracking Hypoxische Signaling in Encapsulated…
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Bioengineering

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JoVE Journal Bioengineering
Tracking Hypoxische Signaling in Encapsulated Zellaggregate
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Tracking Hypoxische Signaling in Encapsulated Zellaggregate

Article DOI: 10.3791/3521-v • 09:14 min • December 16th, 2011
December 16th, 2011 •
1, 1, 2
1Biomedical Engineering Program, University of South Carolina, 2Chemical Engineering Department, University of South Carolina

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Ein Verfahren zur Foto-Verkapselung von Zellen in einer vernetzten PEG-Hydrogel beschrieben. Hypoxische Signalisierung innerhalb gekapselt murine Insulinom (MIN6) Aggregate verfolgt wird mit einem fluoreszierenden Marker-System. Dieses System ermöglicht serielle Untersuchung von Zellen in einem Hydrogel Gerüst und Korrelation von hypoxischen Signalisierung mit Veränderungen in der Zelle Phänotyp.

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Bioengineering Zell-Verkapselung PEG Zellaggregation Hypoxie Insulinsekretion Fluoreszenz-Imaging
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