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Journal / Bioengineering / Sporing hypoksisk Signaling innen Encapsulated…
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Bioengineering

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JoVE Journal Bioengineering
Sporing hypoksisk Signaling innen Encapsulated Cell tilslag
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Sporing hypoksisk Signaling innen Encapsulated Cell tilslag

Article DOI: 10.3791/3521-v • 09:14 min • December 16th, 2011
December 16th, 2011 •
1, 1, 2
1Biomedical Engineering Program, University of South Carolina, 2Chemical Engineering Department, University of South Carolina

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En metode for foto-innkapsling av celler i en krysskoblet PEG hydrogel er beskrevet. Hypoksisk signalering innenfor innkapslet murine insulinoma (MIN6) aggregater spores ved hjelp av et fluoriserende fargestoff system. Dette systemet tillater seriell undersøkelse av celler i en hydrogel stillas og korrelasjon av hypoksisk signalering med endringer i celle fenotype.

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Bioteknologi Cell innkapsling PEG celle aggregering hypoksi insulin sekresjon fluorescerende bildebehandling
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