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JoVE Journal Neuroscience
Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
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Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays

Article DOI: 10.3791/3600-v • 11:08 min • December 20th, 2011
December 20th, 2011 •
1, 1
1Department of Biological Sciences, Purdue University

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We demonstrate how to dissect and culture chick E4 statoacoustic ganglion and E6 spinal cord explants. Explants are cultured under serum-free conditions in 3D collagen gels for 24 hours. Neurite responsiveness is tested with growth factor-supplemented medium and with protein-coated beads.

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Dissection Culture Chick Statoacoustic Ganglion Spinal Cord Explants Collagen Gels Neurite Outgrowth Assays Sensory Organs Inner Ear SAG Neurons Axon Guidance Cues Survival Factors Otic Axons Misrouting Growth Factors Neurotrophin-3 (NT-3) Brain-derived Neurotrophic Factor (BDNF) Transgenic Animals In Vitro Method Responsiveness Of SAG Neurites Soluble Proteins Morphogens Neuron Survival Embryonic Day 4 (E4) Three-dimensional Collagen Gels
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