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Journal / Biology / Isolation of Rat Portal Fibroblasts by In situ…
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Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion

Article DOI: 10.3791/3669-v • 07:39 min • June 29th, 2012
June 29th, 2012 •
1, 2, 2, 2
1Division of Gastroenterology, Hepatology & Nutrition, Department of Pediatrics, The Children's Hospital of Philadelphia, 2Department of Medicine, University of Pennsylvania

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Summary
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A technique for isolating portal fibroblasts from rat liver is described. Livers are perfused and digested in situ with collagenase, followed by ex vivo digestion of the liver slurry and size selection of cells. This method provides a pure population of portal fibroblasts without the need for passage in culture.

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Isolation Rat Portal Fibroblasts In Situ Liver Perfusion Liver Fibrosis Extracellular Matrix Activated Myofibroblasts Hepatic Stellate Cells Bone Marrow Derived Fibroblasts Biliary Fibrosis Biliary Epithelial Injury Scarring In Vitro Study Cell Population Isolation Technique Outgrowth Technique Liver Perfusion Enzymatic Digestion Size Selection Primary Cells
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