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Immunology and Infection

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Intravital Imaging of the Mouse Popliteal Lymph Node

Article DOI: 10.3791/3720-v • 08:41 min • February 8th, 2012
February 8th, 2012 •
1, 1, 1, 2
1Department of Pediatrics, Case Western Reserve University, 2Department of Pediatrics, Pathology and Biomedical Engineering, Case Western Reserve University

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Summary
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Recent advances in 2-photon microscopy have enabled real-time in situ imaging of live tissues in animal models, thereby enhancing our ability to investigate cellular behavior in both physiologic and pathologic conditions. Here, we outline the preparations required to perform intravital imaging of the mouse popliteal lymph node.

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Intravital Imaging Mouse Popliteal Lymph Node Lymph Nodes Immune Response Immune Cell Interactions Lymphocytes T Cells B Cells NK Cells Dendritic Cells Macrophages Bone Marrow-derived Cellular Elements Self Antigens Foreign Antigens Antigen Receptors Adhesion Molecules Chemokines Stromal Structures Fibro-reticular Network High-resolution Real-time Fluorescent In Vivo Imaging Static Imaging Morphology Position Architecture Lymphocyte Trafficking Dynamic Cell Behavior Intravital Two-photon Laser Scanning
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