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Immunology and Infection

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JoVE Journal Immunology and Infection
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Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood

Article DOI: 10.3791/3741-v • 07:58 min • April 16th, 2012
April 16th, 2012 •
1, 1
1Department of Internal Medicine, Yale University School of Medicine

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We describe use of ImageStream technology (www.amnis.com), which combines quantitative flow cytometry with simultaneous high-resolution digital imaging, to quantify cellular mechanisms of primary immune cells from well-defined patient cohorts. Our studies provide a blueprint for translational investigations to quantify lineage specific cellular responses in small samples from subject cohorts.

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Quantitative Imaging Lineage-specific Toll-like Receptor-mediated Signaling Monocytes Dendritic Cells Small Samples Human Blood Immune Status Infection Disease Severity Outcome Aging Viral Infections Bacterial Infections Vaccines Humoral Immune Responses Cell-mediated Immune Responses Toll-like Receptors (TLRs) Innate Immune System Antimicrobial Host Defense Responses Peripheral Blood Monocytes Elderly Donors Dendritic Cells (DCs) Antigen-presenting Cells Innate Immunity Adaptive Immunity Dysregulation Of TLR3 Macrophages IFN Production West Nile Virus Infection Immunosenescence Therapeutic Intervention
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