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JoVE Journal Neuroscience
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A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

Article DOI: 10.3791/3742-v • 09:50 min • March 7th, 2012
March 7th, 2012 •
1, 1, 2,3, 1, 1,4
1Department of Physics, University of California, San Diego, 2Department of Engineering Science and Mechanics, Pennsylvania State University, 3Department of Neurosurgery, Pennsylvania State University, 4Section of Neurobiology, University of California, San Diego

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Summary
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We present a method to form an imaging window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This method is well suited for longitudinal studies of blood flow, cellular dynamics, and cell/vascular structure using two-photon microscopy.

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Polished And Reinforced Thinned-skull Window Long-term Imaging Mouse Brain In Vivo Imaging Cortical Function Optical Access Intracranial Environment PoRTS Window Optical Clarity Bone Regrowth Inhibition Light Scattering Reduction Inflammation Reduction Two-photon Laser Scanning Microscopy Cerebral Blood Flow Cellular Function Anesthetized Preparations Awake Preparations Optogenetics Blood Flow Disruption
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