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En polert og forsterket tynnet-skalle Window for langsiktig Imaging av Mouse Brain
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En polert og forsterket tynnet-skalle Window for langsiktig Imaging av Mouse Brain

Article DOI: 10.3791/3742-v • 09:50 min • March 7th, 2012
March 7th, 2012 •
1, 1, 2,3, 1, 1,4
1Department of Physics, University of California, San Diego, 2Department of Engineering Science and Mechanics, Pennsylvania State University, 3Department of Neurosurgery, Pennsylvania State University, 4Section of Neurobiology, University of California, San Diego

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Vi presenterer en metode for å danne en avbildning vindu i musen hodeskallen som spenner millimeter og er stabil i flere måneder uten betennelse i hjernen. Denne metoden egner seg godt for longitudinelle studier av blodstrøm, cellulære dynamikk, og celle / vaskulær struktur ved hjelp av to-foton mikroskopi.

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Neuroscience kranial vindu kraniotomi to-foton mikroskopi blodstrøm dendrite optogenetics cortex kapillær microglia kronisk mus
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