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Journal / Immunology and Infection / Avbildning av HIV-1 kuvert-inducerad Virologiskt…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Avbildning av HIV-1 kuvert-inducerad Virologiskt Synapse och signalering av syntetiska lipiddubbelskikt
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
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Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
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Avbildning av HIV-1 kuvert-inducerad Virologiskt Synapse och signalering av syntetiska lipiddubbelskikt

Article DOI: 10.3791/3757-v • 11:46 min • March 8th, 2012
March 8th, 2012 •
*1,2, *2,3, 1,2, 1,2, 2, 1,4
1Department of Pathology, New York University Langone School of Medicine, 2Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, 3Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 4Veteran Affairs New York Harbor Healthcare System
* These authors contributed equally

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Click here for the English version.

Den här artikeln beskrivs en metod för att visualisera bildandet av en HIV-1 hölje-inducerad virologisk synaps på glas som stöds plana dubbelskikten genom total inre reflektion fluorescens (TIRF) mikroskopi. Metoden kan även kombineras med immunofluorescensfärgning att upptäcka aktivering och omfördelning av signalmolekyler som inträffar under HIV-1 hölje-inducerad virologiska synaps formation.

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Immunologi 61 Nummer TIRF mikroskopi plana dubbellager HIV kuvert virologiska synaps
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