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Journal / Immunology and Infection / Imaging di HIV-1 Envelope indotta Synapse…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Imaging di HIV-1 Envelope indotta Synapse virologica e segnalazione sui doppi strati lipidici sintetici
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Imaging di HIV-1 Envelope indotta Synapse virologica e segnalazione sui doppi strati lipidici sintetici

Article DOI: 10.3791/3757-v • 11:46 min • March 8th, 2012
March 8th, 2012 •
*1,2, *2,3, 1,2, 1,2, 2, 1,4
1Department of Pathology, New York University Langone School of Medicine, 2Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, 3Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 4Veteran Affairs New York Harbor Healthcare System
* These authors contributed equally

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Questo articolo descrive un metodo per visualizzare la formazione di una sinapsi HIV-1 busta-indotta virologici vetro bistrati planari supportate da fluorescenza riflessione interna totale (TIRF) microscopia. Il metodo può anche essere combinato con immunofluorescenza per rilevare l'attivazione e ridistribuzione di molecole di segnalazione che si verificano durante HIV-1 busta indotta la formazione di sinapsi virologica.

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Immunology Numero 61 microscopia TIRF doppio strato planare busta HIV sinapsi virologica
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