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Journal / Immunology and Infection / Scanning af HIV-1 Envelope-induceret Virologisk…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Scanning af HIV-1 Envelope-induceret Virologisk Synapse og signaler på syntetiske lipiddobbeltlagenes
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Scanning af HIV-1 Envelope-induceret Virologisk Synapse og signaler på syntetiske lipiddobbeltlagenes

Article DOI: 10.3791/3757-v • 11:46 min • March 8th, 2012
March 8th, 2012 •
*1,2, *2,3, 1,2, 1,2, 2, 1,4
1Department of Pathology, New York University Langone School of Medicine, 2Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, 3Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 4Veteran Affairs New York Harbor Healthcare System
* These authors contributed equally

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Denne artikel beskriver en fremgangsmåde til at visualisere dannelse af en HIV-1 envelope-induceret virologisk synapse på glas understøttet plane dobbeltlag ved total intern refleksion fluorescens (TIRF) mikroskopi. Fremgangsmåden kan også kombineres med immunfluorescensfarvning at detektere aktivering og omfordeling af signalmolekyler, der opstår under HIV-1 envelope-induceret virologiske synapsedannelse.

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Immunologi TIRF mikroskopi plane dobbeltlag HIV envelope virologiske synapse
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