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Isolering af brystepitelceller celler fra tre-dimensionel Blandet-celle klumpformet Co-kultur
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Isolering af brystepitelceller celler fra tre-dimensionel Blandet-celle klumpformet Co-kultur

Article DOI: 10.3791/3760-v • 07:27 min • April 30th, 2012
April 30th, 2012 •
1, 1
1Molecular Oncology Research Institute, Department of Medicine, Tufts Medical Center

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En simpel fremgangsmåde er beskrevet til at analysere virkningerne af væv-fibroblaster på tilknyttede epitelceller. Kombinationen af ​​denne fremgangsmåde og tredimensionale vævskultur kan lette analyse af celler efter isolering fra 3D. Teknikken kan anvendes til celler af forskellige maligne potentiale, så systematisk undersøgelse af virkningerne af tumorassocierede stromata på tumorceller.

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Molecular Biology tumormikromiljøet ekstracellulær matrix tredimensionale co-kultur kugleformet blandet-celle cellekultur
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