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Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
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Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

Article DOI: 10.3791/3788-v • 11:29 min • October 3rd, 2012
October 3rd, 2012 •
1, 1, 1,2
1Department of Pathology, National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine, 2Department of Neurology, National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine

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Summary
Automatic Translation

A new species of cellular prion protein (PrPC) has recently been identified in uninfected human brains using the methods described here. These methods can be used to isolate various PrP species, while some of them are also useful in isolating other misfolded protein aggregates from human brains.

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Soluble PrP Oligomers Insoluble PrP Oligomers Normal Human Brain PrPC PrPSc Detergent-soluble Proteinase K (PK)-digestion Detergent-insoluble Aggregates Conformational Transition α-helical Structures β-sheet Structures Endogenous PrPSc Intermediate PrP Silent Prion Biophysical Approaches Biochemical Approaches Ultracentrifugation Sucrose Step Gradient Sedimentation Size Exclusion Chromatography IPrP Enrichment Gene 5 Protein (g5p) PK-treatment
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