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Journal / Immunology and Infection / Engenharia e Evolução da Synthetic vírus…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Engenharia e Evolução da Synthetic vírus adeno-associado (AAV) Gene vetores de terapia através de DNA Família Shuffling
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A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells
Journal (Immunology and Infection)
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Engenharia e Evolução da Synthetic vírus adeno-associado (AAV) Gene vetores de terapia através de DNA Família Shuffling

Article DOI: 10.3791/3819-v • 21:56 min • April 2nd, 2012
April 2nd, 2012 •
*1, *1, 2, 1, 1, 1, 1
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University
* These authors contributed equally

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Nós demonstramos a base técnica para projetar e evoluir molecularmente sintéticos virais adeno-associados (AAV) vetores de terapia genética através de DNA da família baralhar. Além disso, fornecer orientações gerais e exemplos representativos para a selecção ea análise de individuais cápsides quiméricos com propriedades melhoradas sobre células alvo em cultura ou em ratinhos.

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Immunology Edição 62 vírus adeno-associado AAV a terapia genética biologia sintética vector virai a evolução molecular o DNA baralhar
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