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Immunology and Infection

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JoVE Journal Immunology and Infection
Eine primäre Neuron Culture System zur Erforschung des Herpes Simplex Virus Latenz und Reaktivierung
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Eine primäre Neuron Culture System zur Erforschung des Herpes Simplex Virus Latenz und Reaktivierung

Article DOI: 10.3791/3823-v • 12:22 min • April 2nd, 2012
April 2nd, 2012 •
1, 1, 2, 3, 2,4,5,6,7, 1, 1
1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine

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Das Protokoll beschreibt eine effiziente und reproduzierbare Modellsystem zur Untersuchung Herpes-simplex-Virus Typ 1 (HSV-1) Latenz und Reaktivierung. Der Test verwendet homogene sympathischen Neuronen Kulturen und ermöglicht die molekulare Analyse der Virus-Neuron-Wechselwirkungen unter Verwendung einer Vielzahl von Anwendungen, wie RNA-Interferenz und Expression von rekombinanten Proteinen.

Tags

Immunologie Neuron Zellkultur Herpes Simplex Virus (HSV) Molekularbiologie Virologie
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