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Neuroscience

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JoVE Journal Neuroscience
Enkelt-celle Profilering af at udvikle og Ældre retinaneuroner
Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo
Journal (Neuroscience)
Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
Journal (Neuroscience)
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
 
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Enkelt-celle Profilering af at udvikle og Ældre retinaneuroner

Article DOI: 10.3791/3824-v • 10:20 min • April 19th, 2012
April 19th, 2012 •
1, 1
1Department of Genetics, Development and Cell Biology, Neuroscience Program, Iowa State University

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En fremgangsmåde til isolation af enkelte retinale celler og efterfølgende amplifikation af de cDNA'er beskrevet. Encellede transcriptomics viser graden af ​​cellulær heterogenitet er til stede i et væv og afdækker nye markørgener for sjældne cellepopulationer. Den ledsagende protokol kan tilpasses mange forskellige celletyper.

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Neuroscience Single-celler transcriptomics genekspression celletype markører retina neuroner genetik
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