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Biology

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快速高密度的人类胚胎干细胞培养成纤维细胞去除
 
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快速高密度的人类胚胎干细胞培养成纤维细胞去除

Article DOI: 10.3791/3951-v 07:12 min October 28th, 2012
October 28th, 2012

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Summary

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Click here for the English version.

尽管转型期文化无饲养层条件下的不断努力,人类胚胎干细胞(胚胎干细胞)的推导和文化仍然在很大程度上依赖于文化与的小鼠胚胎馈线(MEF中)。在这里,我们展示了一种新的方法,快速去除饲养者从人类胚胎干细胞培养实验前。

Tags

细胞生物学,第68,人类胚胎干细胞,细胞培养,细胞分离,十月,细胞纯化,MEF去除,SSEA-4
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