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Rapid Fibroblast fjerning fra High Density Menneskelig embryonale stamceller kulturer
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Rapid Fibroblast fjerning fra High Density Menneskelig embryonale stamceller kulturer

Article DOI: 10.3791/3951-v • 07:12 min • October 28th, 2012
October 28th, 2012 •
1, 1
1Department of Engineering, University of California, Merced

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Til tross for pågående arbeidet med å overgangen kulturer til mater-frie forhold, avledning og kultur av menneskelige embryonale stamceller (hESC) fortsatt i stor grad avhengig av co-kulturer med mus embryonale feeders (MEFs). Her viser vi en ny metodikk for raskt å fjerne feeders fra hESC kulturer før eksperimentering.

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Cellular Biology humane embryonale stamceller Cell Culture celleisolasjon okt Cell Rensing MEF fjerning SSEA-4
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