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 JoVE Bioengineering

Endothelialized على microfluidics لدراسة التفاعلات الاوعية الدموية الدقيقة في الأمراض الدموية

*1,2,3,4, *1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4

1Department of Pediatrics, Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta, 4Winship Cancer Institute of Emory University

* These authors contributed equally

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Cite this Article: Endothelialized على microfluidics لدراسة التفاعلات الاوعية الدموية الدقيقة في الأمراض الدموية

Myers, D. R., Sakurai, Y., Tran, R., Ahn, B., Hardy, E. T., Mannino, R., et al. Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases. J. Vis. Exp. (64), e3958, doi:10.3791/3958 (2012).

Disclosures: Endothelialized على microfluidics لدراسة التفاعلات الاوعية الدموية الدقيقة في الأمراض الدموية

Materials: Endothelialized على microfluidics لدراسة التفاعلات الاوعية الدموية الدقيقة في الأمراض الدموية

Name Company Catalog Number Comments
blunt point needle OK International 920050-TE Precision TE needle 20 Gauge x 1/2", pink
dextran Sigma-Aldrich 31392
Fibronectin Sigma-Aldrich F0895
Hole puncher (pin vise) Technical Innovations
Human umbilical cord endothelial cells (HUVECs) Lonza CC-2519
Plasma cleaner Plasma PDC-326
Polydimethylsiloxane (PDMS) Fisher Scientific NC9285739 Sylgard 184 Silicone Elastomer KIT
Sigmacote Sigma-Aldrich SL2
SU-8 2025 Microchem Y111069
SU-8 Developer Microchem Y020100
Syringe pump Harvard Apparatus 70-3008 PHD-ULTRA
tubing(larger) Cole-Parmer Instrument Company 06418-02 Tygonreg microbore tubing, 0.020" ID x 0.060" OD
tubing(smaller) Cole-Parmer Instrument Company 06417-11 PTFE microbore tubing, 0.012" ID x 0.030" OD

References: Endothelialized على microfluidics لدراسة التفاعلات الاوعية الدموية الدقيقة في الأمراض الدموية

  1. Mezzano, D., Quiroga, T., & Pereira, J. The Level of Laboratory Testing Required for Diagnosis or Exclusion of a Platelet Function Disorder Using Platelet Aggregation and Secretion Assays. Semin. Thromb. Hemost. 35, 242,254, doi:10.1055/s-0029-1220785 (2009).
  2. Young, E.W.K. & Beebe, D.J. Fundamentals of microfluidic cell culture in controlled microenvironments. Chemical Society Reviews. 39, 1036-1048 (2010).
  3. Young, E.W.K. & Simmons, C.A. Macro- and microscale fluid flow systems for endothelial cell biology. Lab on a Chip. 10, 143-160 (2010).
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  5. Rosano, J., et al. A physiologically realistic in vitro model of microvascular networks. Biomedical Microdevices. 11, 1051-1057, doi:10.1007/s10544-009-9322-8 (2009).
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  9. Rosenbluth, M.J., Lam, W.A., & Fletcher, D.A. Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry. Lab on a Chip. 8, 1062-1070 (2008).
  10. Shelby, J.P., White, J., Ganesan, K., Rathod, P.K., & Chiu, D.T. A microfluidic model for single-cell capillary obstruction by Plasmodium falciparum-infected erythrocytes. Proceedings of the National Academy of Sciences. 100, 14618-14622, doi:10.1073/pnas.2433968100 (2003).
  11. Borenstein, J., et al. Functional endothelialized microvascular networks with circular cross-sections in a tissue culture substrate. Biomedical Microdevices. 12, 71-79, doi:10.1007/s10544-009-9361-1 (2010).
  12. Nesbitt, W.S., et al. A shear gradient-dependent platelet aggregation mechanism drives thrombus formation. Nat. Med. 15, 665-673, http://www.nature.com/nm/journal/v15/n6/suppinfo/nm.1955_S1.html., (2009).
  13. Prabhakarpandian, B., Shen, M.-C., Pant, K., & Kiani, M.F. Microfluidic devices for modeling cell-cell and particle-cell interactions in the microvasculature. Microvascular Research. 82, 210-220, doi:10.1016/j.mvr.2011.06.013 (2011).
  14. Tsai, M., et al. In vitro modeling of the microvascular occlusion and thrombosis that occur in hematologic diseases using microfluidic technology. The Journal of Clinical Investigation. In press, (2011).
  15. Green, D.A., Murphy, W.G., & Uttley, W.S. Haemolytic uraemic syndrome: prognostic factors. Clinical & Laboratory Haematology. 22, 11-14, doi:10.1046/j.1365-2257.2000.00272.x (2000).

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