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Nano-FEM: Protein Lokalisering Bruke Photo-aktivert Lokalisering Mikroskopi og elektronmikroskopi

Article DOI: 10.3791/3995-v • 13:13 min • December 3rd, 2012
December 3rd, 2012 •
1, 1, 1, 1, 1, 1
1Department of Biology, Howard Hughes Medical Institute, University of Utah

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Vi beskriver en metode for å lokalisere fluorescently merket proteiner i elektron mikrografer. Fluorescens er første lokalisert ved hjelp av foto-aktivert lokalisering mikroskopi på supertynne seksjoner. Disse bildene blir deretter justert til elektron mikrografer av den samme delen.

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Molecular Biology cellebiologi genetikk proteomikk Proteiner Protein lokalisering super-oppløsning fluorescens mikroskopi fluorescens elektronmikroskopi nano-FEM EM SEM elektron micrograph bildebehandling
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