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Identifiering av proteinkomplex i
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Identifiering av proteinkomplex i

Article DOI: 10.3791/4057-v • 14:58 min • November 12th, 2012
November 12th, 2012 •
1,2, 1, 1, 1,3, 1,3
1Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, 2Deparment of Biochemistry, Research and Innovation Centre, University of Regina, 3Department of Medical Genetics and Microbiology, University of Toronto

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Affinitetsrening av märkta proteiner i kombination med masspektrometri (truppminor) är en kraftfull metod för systematisk kartläggning av nätverk proteininteraktioner och för undersökning av mekanistiska grunden för biologiska processer. Här beskriver vi en optimerad sekventiell peptid affinitet (SPA) truppminor förfarande utvecklats för bakterien

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Genetik molekylärbiologi medicin biokemi mikrobiologi affinitetsrening, Gramnegativa bakterier cytosoliska proteiner SPA-taggning homolog rekombination masspektrometri protein-interaktion proteinkomplex
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